DNA

Part:BBa_K1039025

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-11)


Bxb1 Invertible Strong Promoter Switch Test Construct

Bxb1 invertible promoter switch (BBa_K1039001) in PB state with gfp gene (BBa_K1039015) on the prefix side of the switch and rfp gene (BBa_J04450) on the suffix side. GFP is produced in this state of the switch.

The invertible promoter is strong promoter J23119.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 749
    Illegal NheI site found at 949
    Illegal NheI site found at 972
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 1872
    Illegal AgeI site found at 1984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal BsaI site found at 781
    Illegal BsaI.rc site found at 1263


[edit]
Categories
Parameters
None